The Little family adopts an spunky boy named Stuart who looks a lot like a mouse. Mr. and Mrs. Little adore their new son, but their older son George isn't so sure what to make of his new brother and devises a plan to get Stuart out of the house.

Fern is a young farm girl who rescues the runt, Wilbur. Wilbur the pig is saved from certain death and Fern visits him every day at her Uncle Homer's farm. But it's Wilbur's friendship with Charlotte the spider that ultimately saves him from the "smoke house." Charlotte's talent for weaving praiseworthy words about Wilbur into her web turns the Zuckerman farm into a tourist attraction.